Cell Based Assays
Bioassay Systems EnzyLight™ ATP Assay Kit. For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism.
For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ATP or a single cell can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM.
Bioassay Systems QuantiFluo™ Ammonia Assay Kit. For quantitative determination of ammonia/ammonium concentration by fluorimetry at 360nm/450nm (compatible on handheld fluorimeter catalog# FL360450).
For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450nm. Samples: Biological (eg urine) and environ-mental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 200 tests. Detection limit: 12 µM.
Bioassay Systems QuantiChrom™ Calcium Assay Kit. For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism.
For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca2+ in 96-well plate assay. Simple and high-throughput. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).
Bioassay Systems EnzyChrom™ Starch Assay Kit. For quantitative determination of starch and evaluation of drug effects on starch metabolism.
For quantitative determination of starch and evaluation of drug effects on starch metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL starch for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological, agriculture, food etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µg/mL.
Bioassay Systems EnzyChrom™ Sialic Acid Assay Kit. For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µM for fluorimetric assays. Simple and convenient. Can detect free sialic acid by addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: OD, FL: 20, 2 µM.
Bioassay Systems EnzyChrom™ Pyruvate Assay Kit. For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
For quantitative determination of pyruvate and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 0.2 µM.
Bioassay Systems QuantiChrom™ Nitric Oxide Assay Kit. For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism.
For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection range 0.6 - 200 µM in 96-well plate. Rapid and reliable. Using an optimized VCl3 reagent, the time required for reduction of nitrate (NO3-) to nitrite (NO2-) is reduced to 10 min at 60°C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day. Method: OD540nm. Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.6 µM.
Revvity Health Sciences Inc ATPlite Luminescence Assay System 96-well, 5000 Assay Points
ATPlite Luminescence Assay System 96-well, 5000 Assay Points
Dojindo Molecular Technologies Inc Glucose Uptake Assay Kit-Green (1 set) , simple measurement of glucose uptake, 200 times more sensitive than 2-NBDG, no more radioactive waste, high cellular retention, Ex=507 nm, Em=518 nm.
Elevated glucose uptake is a marker of tumors, and glucose transporters are important targets in cancer treatment. One common method for evaluating the glucose uptake ability of cells uses radioisotope-labeled glucose. Although this method has been used for many years, it requires special handling facilities and disposal of radioactive materials, at the disadvantage of inconvenience and high cost. 2-NBDG has been used widely to detect cellular glucose uptake, but the sensitivity is poor because of the low fluorescence intensity.Glucose Uptake Probe-Green has resolved those limitations. This probe emits strong green fluorescence (Ex=507 nm, Em=518 nm), allowing for easy detection of glucose uptake over 200 times more sensitive than 2-NBDG by fluorescent microscope, microplate reader, and flow cytometry.The WI Solution in this kit enhances cellular retention of the probe to give more reliable data. Glucose Uptake Assay Kit-Green is qualified for screening purposes.
Medchemexpress LLC HY-15558A 50mg Medchemexpress, Hoechst 33258 (trihydrochloride) CAS:23491-45-4 Purity:>98%
Medchemexpress, HY-15558A 50mg Hoechst 33258 (trihydrochloride) CAS:23491-45-4 Hoechst 33258 trihydrochloride is a fluorescent dyes, which can be used as a cell dye for DNA. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Medchemexpress LLC HY-D0985A 5mg Medchemexpress, TMRE CAS:115532-52-0 Purity:>98%
Medchemexpress, HY-D0985A 5mg TMRE CAS:115532-52-0 TMRE is a mitochondria specific dye (λ ex =550 nm, λ em =575 nm). Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Biolegend PerCP/Cyanine5.5 Annexin V
PerCP/Cyanine5.5 Annexin V Reactivity: All mammalian species; Apps: FC; Size: 100 tests
Biolegend APC Annexin V Apoptosis Detection Kit with PI
APC Annexin V Apoptosis Detection Kit with PI Reactivity: All mammalian species; Apps: FC; Size: 100 tests
